Background of secretion systems there are six different types of secretion systems in bacteria type i, type ii, type iii, type iv, type v, and type vi type i and iv are found in both gramnegative and grampositive bacteria all other types of secretion systems are found only in gramnegative. Towards a complete structural deciphering of type vi secretion 1 system van 1 son nguyen,2, badreddine douzi 14, eric durand 3, alain roussel,2, eric cascales3 and christian cambillau1 the type vi secretion system t6ss is a dynamic nanomachine. Vibrio cholerae, the causative agent of the diarrheal disease cholera, uses the syringelike type vi secretion system t6ss to pierce adjacent cells. Mar 18, 2015 type vi secretion system translocates a phage tail spikelike protein into target cells where it crosslinks actin. The inner tube, topped by the spike complex, is propelled outside of the cell by the contraction of the sheath. Dissecting the bacterial type vi secretion system by a genome. Type 1 and 5 secretion systems and associated toxins. The type vi secretion system t6ss is used by many gramnegative bacteria as a molecular weapon to modulate neighbouring bacterial and eukaryotic cells, thereby affecting the. The type vi secretion system gene clusters contain from 15 to more than 20 genes, two of which, hcp and vgrg, have been shown to be nearly universally secreted substrates of the system. Type vi secretion system protein, burkholderia pseudomallei.
To investigate the role of the t6ss in invasion of intestines already occupied by symbiotic microbes, we genetically engineered v. Type vi secretion system substrates are transferred and reused. The type vi secretion system t6ss represents one of the most recently recognized examples of these. The secretion of one type iv bacterial effector protein is shown to require a conserved cterminal domain and its bacteriolytic activity is neutralized by a cognate immunity protein whose 3d. The role of salmonella typhimurium type three secretion system t3ss and effector proteins in chromatophore cell response. Screening for secretion of the type vi secretion system. Type vi secretion system in pathogenic escherichia coli. The type vi secretion system t6ss mediates interactions between a diverse range of gramnegative bacterial species. Since this systems can transport a variety of different pathogenic factors outside of the bacterial cell and establish the ability to communicate with the environment in which the bacteria live. Recent studies have led to a drastic increase in the number of characterized t6ss effector proteins and produced a more complete and nuanced view of the adaptive significance of the system.
A multipurpose delivery system with a phagelike machinery. Composition of the type vii secretion system membrane. The second type vi secretion system of pseudomonas. Dissecting the bacterial type vi secretion system by a. The h1t6ss secretes three identified toxins that target other bacteria, providing a fitness advantage for p.
The type vi secretion system resembles the bacteriophage structure slight modification of already existing macromolecular complexes and the adaptation of their initial function to achieve a new goal is a common feature in the development of secretion systems. Therefore, t6sss have attracted much attention as a research topic during the past ten years. Inhibition of type vi secretion by an antitssm llama nanobody. Atomic structures of a bactericidal contractile nanotube in. A new front in microbial warfaredelivery of antifungal effectors. Bacteria can use this system to deliver toxic effector proteins in a. Structure and regulation of the type vi secretion system. The genome sequence of the escherichia coli pathotype o157. One such example is the wellstudied type vi secretion system t6ss, which spans both the inner and outer membrane of gramnegative bacteria and injects effectors from the cytoplasm basler, 2015. Mar 12, 2009 the availability of hundreds of bacterial genomes allowed a comparative genomic study of the type vi secretion system t6ss, recently discovered as being involved in pathogenesis. By combining comparative and phylogenetic approaches using more than 500 prokaryotic genomes, we characterized the global t6ss genetic structure in terms of conservation, evolution and genomic organization.
The t6ss type vi secretion system is one of a recent specialized. The type vi secretion system t6ss is widespread in gramnegative bacteria. In all cases, the idea is to use an existing machine, providing access to the external. The availability of hundreds of bacterial genomes allowed a comparative genomic study of the type vi secretion system t6ss, recently discovered as being involved in pathogenesis. The type iii secretion system effector sptp of salmonella enterica serovar typhi rebecca johnson, aalexander byrne, cedric n.
A mechanism for the colonymerger incompatibility is that a donor cell transports effector proteins to a recipient cell in close proximity through the type vi secretion system t6ss apparatus in a contactdependent process, and myxobacteria use the contactdependent mechanism for outer membrane exchange ome 6,7,8,9,10,11,12. Grishinb,c,d, hamid mirzaeic, and kim ortha,c,1 adepartment of molecular biology, bhoward hughes medical institute, cdepartment of biochemistry, and ddepartment of biophysics, university of texas southwestern medical center, dallas, tx 753908816. The role of type vi secretion system effectors in target. Proceedings of the national academy of sciences, 10611, 4154. Grishinb,c,d, hamid mirzaeic, and kim ortha,c,1 adepartment of molecular biology, bhoward hughes medical institute, cdepartment of biochemistry, and ddepartment of biophysics, university of texas. The type vi secretion system t6ss is a nanomachine deployed by many gram negative bacteria as a weapon against eukaryotic hosts or. Learn vocabulary, terms, and more with flashcards, games, and other study tools. Mar 06, 2015 the secretion of one type iv bacterial effector protein is shown to require a conserved cterminal domain and its bacteriolytic activity is neutralized by a cognate immunity protein whose 3d. The type vi secretion system t6ss is a multiprotein complex that catalyses toxin secretion through the bacterial cell envelope of various gramnegative bacteria including important human pathogens. Poisons with a purpose by russell, alistair b and peterson, s. Type vi secretion and antihost effectors request pdf. The type vi secretion system a widespread and versatile. Bacterial type vi secretion system t6ss is a nanomachine that works. By combining comparative and phylogenetic approaches using more than 500 prokaryotic genomes, we characterized the global t6ss genetic structure in terms of conservation, evolution and genomic.
The t6ss is thought to resemble an inverted phage extending outward from the bacterial cell surface. The type vi secretion system t6ss has been identified in several different bacteria, including the plant pathogenpantoea ananatis. Feb 14, 20 john mekalanos and colleagues describe their surprising findings of the bacterial swordplay that occurs when p. The type vi secretion system t6ss delivers effector proteins into both bacterial and eukaryotic targets in a contactdependent manner ho et al. This secretion system has emerged as a key player in regulating the microbial diversity in a population. Protein secretion systems play a pivotal role in bacterial interspecies interaction and virulence 1, 2. Previous in silico analyses described three different t6ss loci present in the pathogenic strain of p. What are the different types of bacterial secretion systems. Omics studies revealed the factors involved in the. The new edition provides insights into some of the most recent advances in the area of bacterial pathogenesis, including. The t6ss facilitates the secretion and injection of toxic effector proteins into host cells, providing a competitive advantage to bacteria encoding this machinery.
T6sss are currently believed to be complex molecular machines which inject effector proteins into target cells and which incorporate a bacteriophagelike cellpuncturing device. Pathogens such as vibrio cholerae and burkholderia pseudomallei use contractile t6sss to translocate protein virulence factors into target eukaryotic cells 2,3, and t6sss are also used for interbacterial competition 4. Mougous abstract the type vi secretion system t6ss mediates interactions between a broad range of gramnegative bacterial. Chapter 16 type 1 and 5 secretion systems and associated toxins timothy j. Another way to think of waste products might be as extra materials that the body cant use anymore. Jul 21, 2015 protein secretion systems play a pivotal role in bacterial interspecies interaction and virulence 1, 2. While often reported that the t6ss was discovered in 2006 by researchers studying the causative agent of cholera, vibrio cholerae, the first.
John mekalanos and colleagues describe their surprising findings of the bacterial swordplay that occurs when p. The bacterial type vi secretion system t6ss is a secretory apparatus encoded by many gramnegative bacteria. Vfdb secretion system virulence factors in aeromonas. Type iii secretion systems t3sss are employed by gramnegative bacteria to deliver effector proteins into the cytoplasm of infected host cells. Since extra and excretion begin with the same letters, it should be fairly easy to remember that excretion refers to expelling waste substances from the body. The type iv secretion system t4ss is used by gramnegative bacteria to translocate protein and dna substrates across the cell envelope and into target cells. Structural analysis of these and other proteins in this system bear a striking resemblance to the tail spike of the t4 phage, and the activity of the system is. A type vi secretion system effector delivery mechanism. The vibrio cholerae type vi secretion system can modulate. Of the known secretion systems in gramnegative bacteria, the type vi secretion system t6ss enables bacteria to compete with both eukaryotic and prokaryotic species through delivery of toxic effectors 2 4. The type vi secretion system t6ss is a multiprotein machine that uses a springlike mechanism to inject effectors into target cells. Mar 26, 2015 the type vi secretion system t6ss is a multiprotein complex that catalyses toxin secretion through the bacterial cell envelope of various gramnegative bacteria including important human pathogens. Apr 17, 2018 vibrio cholerae, the causative agent of the diarrheal disease cholera, uses the syringelike type vi secretion system t6ss to pierce adjacent cells. The t6ss components and effectors in whole cell and during secretion assays were performed as described.
Proceedings of the national academy of sciences of the united states of america, 104, 15508155. Molecular characterization of a functional type vi secretion system from a clinical isolate of aeromonas hydrophila. Contractile ejection systems are ubiquitous in bacteria 1. Myovirus bacteriophages, exemplified by phage t4, use a similar contractile machine to translocate dna and. Most of the conserved protein components that comprise the t6ss apparatus itself have been identified and. Structure and activity of the type vi secretion system. The type vi secretion system t6ss mediates interactions between a broad range of gramnegative bacterial species. Henderson, university of birmingham, birmingham, uk introduction pathogenic escherichia coli can cause disease in a variety of locations in the human body.
Towards a complete structural deciphering of type vi. To understand the conditions that induce expression of the t4ss in xcc, we analyzed, in vitro and book. Russell ab, peterson sb, mougous jd 2014 type vi secretion system effectors. It is structurally related to the t4 bacteriophage tail tube protein gp19. The plantassociated microbe gene ontology pamgo consortium has been developing standardized terms for describing biological processes and cellular components that play. The type vi secretion system t6ss is a nanomachine deployed by many gramnegative bacteria as a weapon against eukaryotic hosts or prokaryotic competitors. However, no specific substrates or defined roles have been. Bacterial type vi secretion systems t6sss function as contractile nanomachines to puncture target cells and deliver lethal effectors. Review architecture and assembly of the type vi secretion system. Hcp family proteins secreted via the type vi secretion system coordinately regulate escherichia coli k1 interaction with human brain microvascular endothelial cells.
Established microbial colonies can survive type vi secretion assault. The type vi secretion system is the most recently discovered of the six mechanisms for effector secretion elucidated in gramnegative bacteria, particularly notable for its significant structural homology with t4 bacteriophage. The type vi secretion system t6ss is molecular machine used by a wide range of. Identification of divergent type vi secretion effectors. The type ii secretion pathway is a twostep process dedicated to the secretion of folded andor oligomeric exoproteins. The type vi secretion system t6ss is molecular machine used by a wide range of gramnegative bacterial species to transport proteins from the interior cytoplasm or cytosol of a bacterial cell across the cellular envelope into an adjacent target cell. Mar 19, 2014 enteropathogenic escherichia coli use a t3ss to deliver effector proteins that result in the creation of the attaching and effacing lesions. Secretion systems are usually classified into 7 groups. This is a really important field of research, as it is through their secretion systems that pathogenic bacteria both attack and infect host cells, so is key to our understanding of bacterial disease.
H7 revealed the existence of a gene cluster encoding components of a second type iii secretion system, the e. It consists of 14 proteins that assemble into three subcomplexes. The genome of pseudomonas aeruginosa pao1 contains three type vi secretion systems t6sss called h1, h2, and h3t6ss. The type vi secretion system t6ss is used by many gramnegative bacteria as a molecular weapon to modulate neighbouring bacterial and eukaryotic cells, thereby affecting the dynamics of. This initial investigation has been extended to include an additional seven sequenced strains of p.
Comparative genomics of type vi secretion systems in. The t6ss is a multicomponent nanomachine analogous to the contractile. R type pyocins are representatives of contractile ejection systems, a class of biological nanomachines that includes, among others, the bacterial type vi secretion system t6ss and contractile bacteriophage tails. It assembles into a bacteriophage taillike structure that can transport effector proteins into the environment or target cells for competitive survival or pathogenesis. The type vi secretion system t6ss delivers protein effectors to diverse cell types including prokaryotic. Lysis of prey cells induced by delivery of lytic effectors is required for efficient transfer of dna from prey to predator. Omics studies revealed the factors involved in the formation. A type vi secretion system effector protein, vgrg1, from aeromonas hydrophila that induces host cell toxicity by adp ribosylation of actin. Type three secretion system often written type iii secretion system and abbreviated ttss or t3ss, also called injectisome is a protein appendage found in several gramnegative bacteria in pathogenic bacteria, the needlelike structure is used as a sensory probe to detect the presence of eukaryotic organisms and secrete proteins that help the bacteria infect them. Type iv secretion system is not involved in infection. The role of salmonella typhimurium type three secretion. Comparative genomics of type vi secretion systems in strains.
Pathogenic mycobacteria can have up to five different t7s systems, designated esx1. Several different types of protein secretion systems exist in gramnegative bacteria that function to translocate proteins outside of their cells, into the extracellular milieu, and sometimes into adjacent prokaryotic or eukaryotic cells. Type vi secretion delivers bacteriolytic effectors to target. T6ss effectors have been identified by a variety of approaches. Zhou y, tao j, yu h, ni j, zeng l, teng q, kim ks, zhao gp, guo x, yao y. Atomic structures of a bactericidal contractile nanotube. Marker for type vi secretion system effectors dor salomona, lisa n. Identification of divergent type vi secretion effectors using. Type vi protein secretion systems t6sss are specialized transport apparatus which can target both eukaryotic and prokaryotic cells and play key roles in hostpathogenmicrobiota interactions. In the 10 years since the discovery of the t6ss, much has been learned about the structure and function of this versatile protein secretion apparatus. Type i, ii, iii, iv, v, vi, vi and chaperons navigating the pathways are also a part of the system. Jul 20, 2011 the type vi secretion system t6ss is a proteinexport machine that is present in about onequarter of all sequenced bacteria. Numerous studies reveal that the chromatophore cell type, erythrophore, can rapidly detect, by aggregating their pigment organelles, several food associated bacterial pathogens, including.
The injection apparatus is composed of a baseplate on which is built a contractile tail tubesheath complex. For the secretion of proteins across this cell envelope, mycobacteria employ the recently discovered transport pathway known as type vii secretion t7s abdallah et al. The type vi secretion system t6ss is a proteinexport machine that is present in about onequarter of all sequenced bacteria. About frontiers institutional membership books news frontiers social media. Type iv secretion system is not involved in infection process. In this simplified view only the basics of each secretion system are sketched. The type vi secretion system t6ss is the most recently described of the gramnegative bacterial secretion systems and is widely distributed amongst diverse species. Type vi secretion delivers bacteriolytic effectors to. Enteropathogenic escherichia coli use a t3ss to deliver effector proteins that result in the creation of the attaching and effacing lesions.
683 922 811 1450 1412 669 741 1016 54 1507 896 746 28 232 389 1139 127 189 197 1595 312 1320 1370 620 163 202 1511 1356 413 1552 696 822 730 724 42 192 1030 292 907 686